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Servicebio Inc sds page protein loading buffer
Sds Page Protein Loading Buffer, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+loading/buffer+loading/pmc13265561-92-8-12
Average 86 stars, based on 1 article reviews
sds page protein loading buffer - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Polyacrylamide Gel Electrophoresis:

Article Title: Identification of novel protein biomarkers for knee osteoarthritis by integrating human plasma proteome: evidence from Mendelian randomization and preliminary in vitro investigation
Article Snippet: TA BCA protein assay kit (Cat. no. G2026, Servicebio, China) was used to measure protein concentration in the supernatant. .. The protein solution was mixed with a 5 × sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) protein loading buffer (Cat. no. G2013, Servicebio, China) at a ratio of 4:1 and then heated in a 95 °C water bath for 10 min to denature the proteins, which were then separated by SDS-PAGE using a fast gel preparation kit (Cat. no. G2003, Servicebio, China) and transferred onto polyvinylidene fluoride membranes. .. The membranes were blocked with 5% non-fat milk for 1 h and incubated overnight at 4 °C with primary antibodies: rabbit anti-NF-κB p65 (phospho S536) (1:2,000; Cat. no. ab239882, Abcam, UK), mouse anti-PRTN3 (1:1,000; Cat. no. sc-74534, Santa Cruz Biotechnology, USA), mouse anti-MPO (1:1,000; Cat. no. sc-390109, Santa Cruz Biotechnology, USA), and rabbit anti-GAPDH (1:2,000; Cat. no. GB11002, Servicebio, China).

SDS Page:

Article Title: Identification of novel protein biomarkers for knee osteoarthritis by integrating human plasma proteome: evidence from Mendelian randomization and preliminary in vitro investigation
Article Snippet: TA BCA protein assay kit (Cat. no. G2026, Servicebio, China) was used to measure protein concentration in the supernatant. .. The protein solution was mixed with a 5 × sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) protein loading buffer (Cat. no. G2013, Servicebio, China) at a ratio of 4:1 and then heated in a 95 °C water bath for 10 min to denature the proteins, which were then separated by SDS-PAGE using a fast gel preparation kit (Cat. no. G2003, Servicebio, China) and transferred onto polyvinylidene fluoride membranes. .. The membranes were blocked with 5% non-fat milk for 1 h and incubated overnight at 4 °C with primary antibodies: rabbit anti-NF-κB p65 (phospho S536) (1:2,000; Cat. no. ab239882, Abcam, UK), mouse anti-PRTN3 (1:1,000; Cat. no. sc-74534, Santa Cruz Biotechnology, USA), mouse anti-MPO (1:1,000; Cat. no. sc-390109, Santa Cruz Biotechnology, USA), and rabbit anti-GAPDH (1:2,000; Cat. no. GB11002, Servicebio, China).

Article Title: Identification and functional characterization of plasma exosomal miR-618 as a novel biomarker in recurrent depressive disorder
Article Snippet: The supernatant contained total protein who’s concentration was determined using the BCA Protein Concentration Assay Kit (LABLEAD, China). .. The remaining protein samples were mixed with 5× SDS-PAGE protein loading buffer (Servicebio, China) at a 4:1 (v/v) ratio and heat-denatured at 95 °C for 5 min using a metal heating block. .. 40 μg of protein was separated on a 10% SDS-PAGE gel (pre-cast using a One Step PAGE Gel Super Fast Preparation Kit, Meilun, China) by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under constant voltage (200 V) for 45 min, followed by being electrophoretically transferred to a polyvinylidene fluoride (PVDF) membrane (Immobilon, Germany) at 400 mA for 25 min. After incubation for 1 h at room temperature with Tris-Buffered Saline with Tween 20 (TBST, Servicebio, China) containing 5% Bovine Serum Albumin (Beyotime, China), the PVDF membrane was rinsed three times with TBST for 10 min each, followed by overnight incubation at 4 °C with the following primary antibody: CD9 Recombinant Rabbit Monoclonal Antibody (clone NO. SA35-08, Cat# ET1601-9, 1:1,000, HUABIO, China), CD63 Recombinant Rabbit Monoclonal Antibody (clone NO. SY21-02, Cat# ET1607-2, 1:500, HUABIO, China) and TSG101 Recombinant Rabbit Monoclonal Antibody (clone NO. JJ0900, Cat# ET1701-59, 1:2000, HUABIO, China).

Article Title: Dysregulated interferon signaling and hyperinflammation upon FCGBP knockdown in goat bronchial epithelial cells infected with Pasteurella multocida
Article Snippet: Following 48 h, cells were lysed using IP lysis buffer (Servicebio Technology Co., Ltd., China). .. Supernatants were collected after centrifugation, supplemented with 5× SDS-PAGE Protein Loading Buffer (Servicebio Technology Co., Ltd., China), and boiled in a metal bath at 96 °C for 10 min. ..

Multiple Displacement Amplification:

Article Title: Protective Effect of Gastrodia elata Polysaccharide GEP-2 Against Oxidative Stress in Intestinal Epithelial NCM460 Cells.
Article Snippet: H2O2 (C17736142) was sourced from Macklin Biochemical Co., Ltd. (Shanghai, China). .. Assay kits for MDA (G4300), T-AOC (G4313), T-SOD (G4306), CAT (G4307), and GSH (G4310), 5 × SDS protein loading buffer (PE0020), primary/secondary antibody stripping buffer (P0025), fetal bovine serum (BL205B), PBS (C8136), 0.25% trypsin–EDTA solution (C8019), Cell Counting Kit-8 (C8022), ROS detection kit (S0033S), and JC-1 assay kit (C2006), RIPA lysis buffer (G2002), PMSF (G2008-1 mL), phosphatase inhibitor cocktail (G2007-1 mL), and 50 × protease inhibitor cocktail (G2006-250 μL) were also provided by Servicebio Technology Co., Ltd. (Wuhan, China). .. DMEM (6125525) came from Gibco (Grand Island, NY, USA).

Stripping Membranes:

Article Title: Protective Effect of Gastrodia elata Polysaccharide GEP-2 Against Oxidative Stress in Intestinal Epithelial NCM460 Cells.
Article Snippet: H2O2 (C17736142) was sourced from Macklin Biochemical Co., Ltd. (Shanghai, China). .. Assay kits for MDA (G4300), T-AOC (G4313), T-SOD (G4306), CAT (G4307), and GSH (G4310), 5 × SDS protein loading buffer (PE0020), primary/secondary antibody stripping buffer (P0025), fetal bovine serum (BL205B), PBS (C8136), 0.25% trypsin–EDTA solution (C8019), Cell Counting Kit-8 (C8022), ROS detection kit (S0033S), and JC-1 assay kit (C2006), RIPA lysis buffer (G2002), PMSF (G2008-1 mL), phosphatase inhibitor cocktail (G2007-1 mL), and 50 × protease inhibitor cocktail (G2006-250 μL) were also provided by Servicebio Technology Co., Ltd. (Wuhan, China). .. DMEM (6125525) came from Gibco (Grand Island, NY, USA).

CCK-8 Assay:

Article Title: Protective Effect of Gastrodia elata Polysaccharide GEP-2 Against Oxidative Stress in Intestinal Epithelial NCM460 Cells.
Article Snippet: H2O2 (C17736142) was sourced from Macklin Biochemical Co., Ltd. (Shanghai, China). .. Assay kits for MDA (G4300), T-AOC (G4313), T-SOD (G4306), CAT (G4307), and GSH (G4310), 5 × SDS protein loading buffer (PE0020), primary/secondary antibody stripping buffer (P0025), fetal bovine serum (BL205B), PBS (C8136), 0.25% trypsin–EDTA solution (C8019), Cell Counting Kit-8 (C8022), ROS detection kit (S0033S), and JC-1 assay kit (C2006), RIPA lysis buffer (G2002), PMSF (G2008-1 mL), phosphatase inhibitor cocktail (G2007-1 mL), and 50 × protease inhibitor cocktail (G2006-250 μL) were also provided by Servicebio Technology Co., Ltd. (Wuhan, China). .. DMEM (6125525) came from Gibco (Grand Island, NY, USA).

Lysis:

Article Title: Protective Effect of Gastrodia elata Polysaccharide GEP-2 Against Oxidative Stress in Intestinal Epithelial NCM460 Cells.
Article Snippet: H2O2 (C17736142) was sourced from Macklin Biochemical Co., Ltd. (Shanghai, China). .. Assay kits for MDA (G4300), T-AOC (G4313), T-SOD (G4306), CAT (G4307), and GSH (G4310), 5 × SDS protein loading buffer (PE0020), primary/secondary antibody stripping buffer (P0025), fetal bovine serum (BL205B), PBS (C8136), 0.25% trypsin–EDTA solution (C8019), Cell Counting Kit-8 (C8022), ROS detection kit (S0033S), and JC-1 assay kit (C2006), RIPA lysis buffer (G2002), PMSF (G2008-1 mL), phosphatase inhibitor cocktail (G2007-1 mL), and 50 × protease inhibitor cocktail (G2006-250 μL) were also provided by Servicebio Technology Co., Ltd. (Wuhan, China). .. DMEM (6125525) came from Gibco (Grand Island, NY, USA).

Protease Inhibitor:

Article Title: Protective Effect of Gastrodia elata Polysaccharide GEP-2 Against Oxidative Stress in Intestinal Epithelial NCM460 Cells.
Article Snippet: H2O2 (C17736142) was sourced from Macklin Biochemical Co., Ltd. (Shanghai, China). .. Assay kits for MDA (G4300), T-AOC (G4313), T-SOD (G4306), CAT (G4307), and GSH (G4310), 5 × SDS protein loading buffer (PE0020), primary/secondary antibody stripping buffer (P0025), fetal bovine serum (BL205B), PBS (C8136), 0.25% trypsin–EDTA solution (C8019), Cell Counting Kit-8 (C8022), ROS detection kit (S0033S), and JC-1 assay kit (C2006), RIPA lysis buffer (G2002), PMSF (G2008-1 mL), phosphatase inhibitor cocktail (G2007-1 mL), and 50 × protease inhibitor cocktail (G2006-250 μL) were also provided by Servicebio Technology Co., Ltd. (Wuhan, China). .. DMEM (6125525) came from Gibco (Grand Island, NY, USA).

Blocking Assay:

Article Title: Identification and functional characterization of plasma exosomal miR-618 as a novel biomarker in recurrent depressive disorder
Article Snippet: The supernatant contained total protein who’s concentration was determined using the BCA Protein Concentration Assay Kit (LABLEAD, China). .. The remaining protein samples were mixed with 5× SDS-PAGE protein loading buffer (Servicebio, China) at a 4:1 (v/v) ratio and heat-denatured at 95 °C for 5 min using a metal heating block. .. 40 μg of protein was separated on a 10% SDS-PAGE gel (pre-cast using a One Step PAGE Gel Super Fast Preparation Kit, Meilun, China) by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under constant voltage (200 V) for 45 min, followed by being electrophoretically transferred to a polyvinylidene fluoride (PVDF) membrane (Immobilon, Germany) at 400 mA for 25 min. After incubation for 1 h at room temperature with Tris-Buffered Saline with Tween 20 (TBST, Servicebio, China) containing 5% Bovine Serum Albumin (Beyotime, China), the PVDF membrane was rinsed three times with TBST for 10 min each, followed by overnight incubation at 4 °C with the following primary antibody: CD9 Recombinant Rabbit Monoclonal Antibody (clone NO. SA35-08, Cat# ET1601-9, 1:1,000, HUABIO, China), CD63 Recombinant Rabbit Monoclonal Antibody (clone NO. SY21-02, Cat# ET1607-2, 1:500, HUABIO, China) and TSG101 Recombinant Rabbit Monoclonal Antibody (clone NO. JJ0900, Cat# ET1701-59, 1:2000, HUABIO, China).

Centrifugation:

Article Title: Dysregulated interferon signaling and hyperinflammation upon FCGBP knockdown in goat bronchial epithelial cells infected with Pasteurella multocida
Article Snippet: Following 48 h, cells were lysed using IP lysis buffer (Servicebio Technology Co., Ltd., China). .. Supernatants were collected after centrifugation, supplemented with 5× SDS-PAGE Protein Loading Buffer (Servicebio Technology Co., Ltd., China), and boiled in a metal bath at 96 °C for 10 min. ..



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Image Search Results


Journal: Frontiers in Oncology

Article Title: Integrating network pharmacology, molecular docking, and experimental validation, this study explores the mechanism of action of Typhonii Rhizoma in colon cancer

doi: 10.3389/fonc.2026.1815506

Figure Lengend Snippet: Experiment-related reagents.

Article Snippet: 5× Protein Loading Buffer (DTT-containing) , Epizyme , 4% Hematoxylin and Eosin Fixative , Solarbio.

Techniques: CCK-8 Assay, Protease Inhibitor, Western Blot, Membrane, Electrophoresis